GRAF Antibody

Images

 
Western Blot: GRAF Antibody [NB100-1327] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected ...read more
Immunohistochemistry-Paraffin: GRAF Antibody [NB100-1327] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GRAF Antibody Summary

Immunogen
Peptide with sequence C-KTGLIPENYVEFL corresponding to C-Terminus according to NP_055886.1, NP_001129080.1.
Specificity
This antibody is expected to recognise both reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARHGAP26
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 90 kDa band observed in human heart lysates (calculated MW of 92.2 kDa band according to NP_055886.1). An additional strong band of unknown identity was also consistently observed at 16 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Colon.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Heart Lysate (NB820-59217)
Publications
Read Publication using NB100-1327.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRAF Antibody

  • FLJ42530
  • GRAFGTPase regulator associated with focal adhesion kinase pp125(FAK)
  • KIAA0621rho GTPase-activating protein 26
  • Oligophrenin-1-like protein
  • OPHN1L1
  • OPHN1LGTPase regulator associated with focal adhesion kinase
  • Rho GTPase activating protein 26
  • Rho-type GTPase-activating protein 26

Background

GTPase regulator associated with the focal adhesion kinase pp125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRAF Antibody (NB100-1327)(1)

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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGAP26
Entrez
Uniprot