GRAF Antibody

Western Blot: GRAF Antibody [NB100-1327] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: GRAF Antibody [NB100-1327] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GRAF Antibody Summary

• Description: Novus Biologicals Goat GRAF Antibody (NB100-1327) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-GRAF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KTGLIPENYVEFL corresponding to C-Terminus according to NP_055886.1, NP_001129080.1.
• Specificity: This antibody is expected to recognise both reported isoforms.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGAP26
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human heart lysates (calculated MW of 92.2 kDa band according to NP_055886.1). An additional strong band of unknown identity was also consistently observed at 16 kDa. This band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Colon.
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GRAF Antibody

• FLJ42530
• GRAFGTPase regulator associated with focal adhesion kinase pp125(FAK)
• KIAA0621rho GTPase-activating protein 26
• Oligophrenin-1-like protein
• OPHN1L1
• OPHN1LGTPase regulator associated with focal adhesion kinase
• Rho GTPase activating protein 26
• Rho-type GTPase-activating protein 26

Background

GTPase regulator associated with the focal adhesion kinase pp125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GRAF Antibody (NB100-1327)(1)

Publication using NB100-1327

• Borkhardt A, Bojesen S, Haas OA et al. The human GRAF gene is fused to MLL in a unique t(5;11)(q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q. Proc Natl Acad Sci U S A 2000-08-01 [PMID: 10908648]

Bioinformatics

• Gene Symbol: ARHGAP26
• Entrez:
  • 23092(Human)
  • 71302(Mouse)
• Uniprot:
  • Q9UNA1(Human)