GPSM2 Antibody

Immunocytochemistry/Immunofluorescence: GPSM2 Antibody [NBP1-85232] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Immunohistochemistry-Paraffin: GPSM2 Antibody [NBP1-85232] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: GPSM2 Antibody [NBP1-85232] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells in molecular layer and granular layer.

Immunohistochemistry-Paraffin: GPSM2 Antibody [NBP1-85232] - Staining of human cerebellum shows strong cytoplasmic and membranous positivity in Purkinje cells as well as in cells in molecular and granular layer.

Immunohistochemistry-Paraffin: GPSM2 Antibody [NBP1-85232] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Product Discontinued

GPSM2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: REVGDKSGELTARLNLSDLQMVLGLSYSTNNSIMSENTEIDSSLNGVRPKLGRRHSMENMELMKLTPEKVQNWNSEILAKQKPLIAKPSAKLLFVNRLKGKKYKTNSSTKVLQDASNSIDHRIPNSQRKISADTIGDEGFFDL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPSM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: ICC/IF reported in scientific literature (PMID: 24996901). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Mouse (88%), Rat (81%). Reactivity reported in scientific literature (PMID: 23027904)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GPSM2 Antibody

• deafness, autosomal recessive 82
• DFNB82
• G-protein signaling modulator 2
• G-protein signalling modulator 2 (AGS3-like, C. elegans)
• G-protein-signaling modulator 2
• LGNPins
• Mosaic protein LGN
• PINS

Background

Heterotrimeric G proteins transduce extracellular signals received by cell surface receptors into integrated cellular responses. GPSM2 belongs to a group of proteins that modulate activation of G proteins (Blumer et al., 2002).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPSM2 Antibody (NBP1-85232)(2)

Publications using NBP1-85232

• Kotak S, Busso C, Gonczy P. NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane. EMBO J 2014-08-18 [PMID: 24996901] (ICC/IF, Human)
• Kotak S, Busso C, Gonczy P et al. Cortical dynein is critical for proper spindle positioning in human cells. J Cell Biol 2012-10-01 [PMID: 23027904]

Bioinformatics

• Gene Symbol: GPSM2