GPSM2 Antibody

Immunocytochemistry/Immunofluorescence: GPSM2 Antibody [NB100-57096] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: GPSM2 Antibody [NB100-57096] - Staining of paraffin embedded Human Testis with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GPSM2 Antibody Summary

• Description: Novus Biologicals Goat GPSM2 Antibody (NB100-57096) is a polyclonal antibody validated for use in IHC, ELISA and ICC/IF. Anti-GPSM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NRLKGKKYKTNSSTK corresponding to internal region according to NP_037428.2.
• Epitope: NRLKGKKYKTNSSTK
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPSM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
• Control:

  GPSM2 Overexpression Lysate
  Human Brain Cerebellum Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPSM2 Antibody

• deafness, autosomal recessive 82
• DFNB82
• G-protein signaling modulator 2
• G-protein signalling modulator 2 (AGS3-like, C. elegans)
• G-protein-signaling modulator 2
• LGNPins
• Mosaic protein LGN
• PINS

Background

Heterotrimeric G proteins transduce extracellular signals received by cell surface receptors into integrated cellular responses. GPSM2 belongs to a group of proteins that modulate activation of G proteins (Blumer et al., 2002).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPSM2 Antibody (NB100-57096)(1)

Publication using NB100-57096

• Yasumi M, Sakisaka T, Hoshino T et al. Direct binding of Lgl2 to LGN during mitosis and its requirement for normal cell division. J Biol Chem 2005-02-25 [PMID: 15632202]

Bioinformatics

• Gene Symbol: GPSM2
• Entrez:
  • 29899(Human)
• Uniprot:
  • NP_037428.2(Human)