GPR81 Antibody (1048103) [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GPR81 Monoclonal Antibody (Catalog # MAB11134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR81 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 1048103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR81 Antibody (1048103) [Unconjugated] Summary

• Immunogen: Synthetic peptide corresponding to the N-term sequences of Human HCAR1/GPR81
• Specificity: Detects human HCAR1/GPFR81 peptide in direct Elisa
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR81 Antibody (1048103) [Unconjugated]

• FKSG80
• G protein-coupled receptor 104
• G protein-coupled receptor 81
• GPR104
• GPR81
• G-protein coupled receptor 104
• G-protein coupled receptor 81
• HCA1
• HCAR1
• LACR1
• lactate receptor 1
• TA-GPCR
• T-cell activation G protein-coupled receptor

Background

Human Hydroxycarboxylic acid receptor 1 (HCAR1), also known as GPR81 and GPR194, is a G-protein coupled receptor (GPCR) thatt acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.