GPR32 Overexpression Lysate (Native)

Images

 
Western Blot: GPR32 Overexpression Lysate (Adult Normal) [NBL1-11274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR32.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GPR32 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209670 and based on accession number NM_001506. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens G protein-coupled receptor 32 (GPR32), mRNA.
Gene
GPR32

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GPR32

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GPR32 Overexpression Lysate (Native)

  • G protein-coupled receptor 32
  • GPR32
  • probable G-protein coupled receptor 32
  • RVDR1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NLS1878
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00084941-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89396
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NLS1317
Species: Hu, Mk, Pm
Applications: Flow, IHC-P
NBP2-47645
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, PAGE, IF
MAB362
Species: Hu
Applications: Flow, CyTOF-ready
NBP2-27422
Species: Hu, Pm
Applications: WB, IHC-P
NBP2-13961
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF9024
Species: Mu, Rt
Applications: WB, IHC
NLS418
Species: Hu
Applications: IHC-P
PP-H4537-00
Species: Hu
Applications: WB, IHC, IP, DirELISA

Publications for GPR32 Lysate (NBL1-11274) (0)

There are no publications for GPR32 Lysate (NBL1-11274).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GPR32 Lysate (NBL1-11274) (0)

There are no reviews for GPR32 Lysate (NBL1-11274). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GPR32 Lysate (NBL1-11274) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GPR32 Products

Bioinformatics Tool for GPR32 Lysate (NBL1-11274)

Discover related pathways, diseases and genes to GPR32 Lysate (NBL1-11274). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GPR32 Lysate (NBL1-11274)

Discover more about diseases related to GPR32 Lysate (NBL1-11274).
 

Pathways for GPR32 Lysate (NBL1-11274)

View related products by pathway.

Research Areas for GPR32 Lysate (NBL1-11274)

Find related products by research area.

Blogs on GPR32

There are no specific blogs for GPR32, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GPR32 Antibody
NBP1-87526

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GPR32 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GPR32
Entrez