GPR3 Antibody

Western Blot: GPR3 Antibody [NBP1-00196] - (0.3ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GPR3 Antibody Summary

• Description: Novus Biologicals Goat GPR3 Antibody (NBP1-00196) is a polyclonal antibody validated for use in WB and ELISA. Anti-GPR3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LQRHLLPASH corresponding to internal region according to NP_005272.1.
• Specificity: This antibody is not expected to cross-react with GPR6 and GPR12.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human brain (hippocampus and frontal cortex) lysates (calculated MW of 35.0 kDa band according to NP_005272.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR3 Antibody

• ACCA
• G protein-coupled receptor 3
• GPR3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR3 Antibody (NBP1-00196)(1)

Publication using NBP1-00196

• Tanaka S, Ishii K, Kasai K et al. Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth. J Biol Chem 2007-04-01 [PMID: 17284443]

Bioinformatics

• Gene Symbol: GPR3
• Entrez:
  • 2827(Human)
  • 14748(Mouse)
  • 266769(Rat)
• Uniprot:
  • NP_005272.1(Human)