GPR12 Antibody (584940) [Unconjugated]

Images

 
GPR12 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
HEK293 human embryonic kidney cell line transfected with (A) Human GRP12 or (B) irrelevant transfectant, and eGFP was stained with Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC
Clone
584940
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

GPR12 Antibody (584940) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human GPR12
Met1-Val334
Accession # P47775
Specificity
Detects human GPR12 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GPR12
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR12 Antibody (584940) [Unconjugated]

  • FLJ18149
  • FLJ97704
  • G protein-coupled receptor 12
  • GPCR12
  • GPCR21
  • GPR12
  • G-protein coupled receptor 12
  • MGC138349
  • MGC138351

Background

GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for GPR12 Antibody (MAB6738) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6738U
Alexa Fluor 405 FAB6738V
Alexa Fluor 488 FAB6738G
Alexa Fluor 594 FAB6738T
Alexa Fluor 647 FAB6738R
Alexa Fluor 700 FAB6738N
Alexa Fluor 750 FAB6738S
Phycoerythrin FAB6738P

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Bioinformatics

Gene Symbol GPR12
Uniprot