GPR17 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR17.

Immunohistochemistry-Paraffin: GPR17 Antibody [NLS4229] - Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunocytochemistry/Immunofluorescence: GPR17 Antibody [NLS4229] - immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.

Immunohistochemistry-Paraffin: GPR17 Antibody [NLS4229] - IHC staining of human brain, neurons and glia.

• Reactivity: Hu, Pm
• Applications: ICC/IF, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPR17 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 16 amino acid peptide from N-terminal extracellular domain of human GPR17.
• Epitope: N-Terminus extracellular domain
• Localization: Integral membrane protein.
• Specificity: Human GPR17. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-16 ug/ml
  • Immunocytochemistry/Immunofluorescence 3-16 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR17 Antibody - BSA Free

• DKFZp686M18273
• G protein-coupled receptor 17
• GPR17
• G-protein coupled receptor 17
• P2Y-like Receptor
• R12
• UDP/CysLT Receptor
• uracil nucleotide/cysteinyl leukotriene receptor

Background

GPR17 is an Orphan-A GPCR with an unknown ligand. GPR17 has been reported to be expressed exclusively in brain as 2.3- and 6.3-kb mRNAs (Blasius et al., 1998). ESTs have been isolated from human brain, heart, melanocyte/uterus/fetal heart, and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR17 Antibody (NLS4229)(2)

Publications using NLS4229

• Yan S, Conley JM, Reilly AM et al. Intestinal Gpr17 deficiency improves glucose metabolism by promoting GLP-1 secretion Cell reports 2022-01-04 [PMID: 34986353] (IF/IHC, Human)
• Paruchuri, S, Jiang, Y, Feng, C et al. Leukotriene E4 activates peroxisome proliferator activated receptor gamma induces prostaglin D2 generatioin by human mast cells. J. Biol. Chem. 2008-04-14 [PMID: 18411276]

Bioinformatics

• Gene Symbol: GPR17
• Entrez:
  • 2840(Human)
• Uniprot:
  • Q13304(Human)