GPR153 Antibody

Immunohistochemistry-Paraffin: GPR153 Antibody [NBP1-02392] - Staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 17 ug/ml.

Product Discontinued

GPR153 Antibody Summary

• Immunogen: Synthetic 16 amino acid peptide from C-terminal cytoplasmic domain of human GPR153.
• Specificity: Human GPR153. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PCDHGA2 (56%), PCDHGA4 (50%).
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR153
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 34 ug/ml
• Application Notes: IHC: NBP1-02392 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Predicted cross-reactivity based on seqeunce identity: Marmoset (88%), Mouse (88%), Bovine (88%), Hamster (88%), Elephant (88%), Bat (81%), Canine (81%), Porcine (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR153 Antibody

• DKFZp762B2210
• G protein-coupled receptor 153
• GPR153
• PGR1
• PGR1G-protein coupled receptor PGR1
• probable G-protein coupled receptor 153

Background

GPR153 is an Orphan-U GPCR with an unknown ligand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR153
• Uniprot:
  • Q6NV75(Human)