GPR12 Antibody (584940) [Phycoerythrin]

HEK293 human embryonic kidney cell line transfected with (A) human GPR12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GPR12 PE-conjugated Monoclonal Antibody (Catalog # FAB6738P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 584940
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

GPR12 Antibody (584940) [Phycoerythrin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GPR12
  Accession # P47775
• Specificity: Detects human GPR12 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR12 Antibody (584940) [Phycoerythrin]

• FLJ18149
• FLJ97704
• G protein-coupled receptor 12
• GPCR12
• GPCR21
• GPR12
• G-protein coupled receptor 12
• MGC138349
• MGC138351

Background

GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P47775()