GPR116 Antibody (1055815) [Unconjugated]

HEK293 human cell line transfected with human GPR116 was stained with either (A) Mouse Anti-Human GPR116 Monoclonal Antibody (Catalog # MAB112481) or (B) Mouse IgG1 Isotype Control (MAB002), followed by APC-conjugated anti-mouse IgG secondary antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1055815
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR116 Antibody (1055815) [Unconjugated] Summary

• Immunogen: E. coli-expressed recombinant Human GPR116 extracellular domain.
  His643-Asn945
  Accession # Q8IZF2
• Specificity: Detects Human GPR116 in Direct ELISAs. In Flow Cytometry, it detects human GPR116 in transfected cells, but not in non-transfected parental cell line.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR116 Antibody (1055815) [Unconjugated]

• FLJ90640
• G protein-coupled receptor 116
• GPR116
• Ig-Hepta homolog
• KIAA0758DKFZp564O1923
• KPG_001
• probable G-protein coupled receptor 116

Background

GPR116, also known as  Adhesion G protein-coupled receptor F5 or ADGRF5, belongs to the LN-TM7 subfamily of the G protein-coupled receptor 2 family, also known as adhesion GPCRs. It exists as a highly glycosylated disulfide-linked dimer at the cell surface. GPR116 may have a role in the regulation of acid-base balance and is also being investigated for it's involvement in adipocyte biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Uniprot