GPR116 Antibody (1055814) [Unconjugated]

GPR116 was detected in immersion fixed paraffin-embedded sections of Human Lung using Mouse Anti-Human GPR116 Monoclonal Antibody (Catalog # MAB11248) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1055814
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR116 Antibody (1055814) [Unconjugated] Summary

• Immunogen: E. coli-expressed recombinant Human GPR116 extracellular domain.
  His643-Asn945
• Specificity: Detects Human GPR116 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR116 Antibody (1055814) [Unconjugated]

• FLJ90640
• G protein-coupled receptor 116
• GPR116
• Ig-Hepta homolog
• KIAA0758DKFZp564O1923
• KPG_001
• probable G-protein coupled receptor 116

Background

GPR116 belongs to the LN-TM7 subfamily of the G protein-coupled receptor 2 family, also known as adhesion GPCRs. It exists as a highly glycosylated disulfide-linked dimer at the cell surface. GPR116 may have a role in the regulation of acid-base balance and is also being investigated for it's involvement in adipocyte biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.