![GPR114 antibody [HL3850] detects GPR114 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPR114 stained by GPR114 antibody [HL3850] (NBP3-48850) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48850_rabbit-gpr114-mab-hl3850-immunocytochemistry-immunofluorescence-1411202518392016.jpg "GPR114 antibody [HL3850] detects GPR114 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPR114 stained by GPR114 antibody [HL3850] (NBP3-48850) diluted at 1:500.Blue: Fluoroshield with DAPI .")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | HL3850 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit GPR114 Antibody (HL3850) (NBP3-48850) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide encompassing a sequence within the Extracellular domain of human GPR114. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ADGRG5 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for GPR114 Antibody (NBP3-48850)Find related products by research area.
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| Gene Symbol | ADGRG5 |
![Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR114 antibody [HL3850] (NBP3-48850) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48850_rabbit-gpr114-mab-hl3850-western-blot-1411202518395417.jpg "Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR114 antibody [HL3850] (NBP3-48850) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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