GnRHR Antibody (F1G4 (same as GNRH03))

Images

 
Western Blot: GnRHR Antibody (F1G4 (same as GNRH03)) [NBP2-45298] - Western Blot Analysis of Human MDA-231 cell lysate using GnRHR Antibody (F1G4 (same as GNRH03)).
Immunohistochemistry-Paraffin: GnRHR Antibody (F1G4 (same as GNRH03)) [NBP2-45298] - Human Ovarian Carcinoma stained with GnRH-Receptor Monoclonal Antibody (F1G4)
Immunofluorescence: GnRHR Antibody (F1G4 (same as GNRH03)) [NBP2-45298] - Immunofluorescence Analysis of Human MCF-7 cells labeling GnRH-R with GnRHR Antibody (F1G4 (same as GNRH03)) followed by Goat anti-mouse ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
F1G4 (same as GNRH03)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

GnRHR Antibody (F1G4 (same as GNRH03)) Summary

Immunogen
A synthetic peptide aa 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) from the N-terminal of human GnRHR.
Localization
Cytoplasmic
Specificity
Recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GNRHR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reactivity Notes

Predicted to react with Porcine, Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml (NBP3-11541).

Alternate Names for GnRHR Antibody (F1G4 (same as GNRH03))

  • GnRH receptor
  • GnRHR
  • GnRH-R
  • GNRHR1
  • gonadotropin-releasing hormone (type 1) receptor 1
  • gonadotropin-releasing hormone receptor
  • GRHR
  • HH7
  • leutinizing-releasing hormone receptor
  • LHRHR
  • LHRHRleutinizing hormone releasing horomone receptor
  • LRHR
  • luliberin receptor
  • Luliberin
  • type I GnRH receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GNRHR