Mouse Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

ELISA: Mouse Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82422] - Samples were spiked with high concentrations of Mouse Glycogen Phosphorylase BB/GPBB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) [NBP2-82422] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Mouse Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Glycogen Phosphorylase BB/GPBB in samples. No Significant cross-reactivity or interference between Mouse Glycogen Phosphorylase BB/GPBB and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.68
• Intra-Assay: CV% < 4.42
• Spike Recovery: 84-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PYGB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Glycogen Phosphorylase BB/GPBB ELISA Kit (Colorimetric)

• Brain glycogen phosphorylase
• EC 2.4.1.1
• Glycogen phosphorylase B
• Glycogen phosphorylase brain form
• Glycogen Phosphorylase Isoenzyme BB
• GPBB
• MGC9213
• Phosphorylase glycogen brain
• PYGB

Background

GPBB is found predominantly in the brain. Glycogen phosphorylase is a key enzyme of glycogenolysis, catalyzing the rate determining step in glycogen degradation. The activity of this enzyme is allosterically activated by AMP and alosterically inhibited by ATP, ADP, and glucose 6 phosphate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Glycogen Phosphorylase BB/GPBB ELISA Kit (NBP2-82422). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PYGB