Glutathione Peroxidase 5 Antibody (5A1)

Western Blot: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Analysis of GPX5 on mouse testis tissue lysate using anti-GPX5 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Staining GPX5 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Analysis of paraffin-embedded mouse testis tissue using anti-GPX5 antibody. Counter stained with hematoxylin.

Western Blot: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Staining GPX5 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Analysis of paraffin-embedded human liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 5 Antibody (5A1) [NBP2-75512] - Analysis of paraffin-embedded mouse liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.

Product Discontinued

Glutathione Peroxidase 5 Antibody (5A1) Summary

• Immunogen: Synthetic peptide within Human Glutathione Peroxidase 5 aa 85-134 / 221. (SwissProt: O75715 Human; SwissProt: P21765 Mouse)
• Localization: Secreted
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GPX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 5 Antibody (5A1)

• EC 1.11.1
• EC 1.11.1.9
• EGLP
• epididymal androgen-related protein
• epididymal secretory glutathione peroxidase
• Epididymis-specific glutathione peroxidase-like protein
• glutathione peroxidase 5 (epididymal androgen-related protein)
• Glutathione peroxidase 5
• GPx-5
• GSHPx-5

Background

Glutathione peroxidase (GPX)/glutathione reductase is part of the hydrogen peroxide scavenging system found within the epididymis in the mammalian male reproductive tract. GPX5 expression is epididymis-specific and the transcript is unique from other GPXs because it contains a deletion resulting in an mRNA that does not contain a selenocysteine (UGA) codon (an unusual amino acid present in other GPXs). This deletion also renders the mRNA incapable of encoding an active GPX isoenzyme. For this reason, GPX5 is selenium-independent and has very little activity towards hydrogen peroxide or organic hydroperoxides. GPX5, which is bound to the acrosome of sperm, may act to protect sperm from premature acrosome reaction in the epididymis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX5