Glutathione Peroxidase 1/GPX1 Antibody (JF0944)

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Staining Glutathione Peroxidase 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Staining Glutathione Peroxidase 1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Staining Glutathione Peroxidase 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Analysis of paraffin-embedded human kidney tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Analysis of paraffin-embedded human liver tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody (JF0944) [NBP2-66874] - Analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.

Product Discontinued

Glutathione Peroxidase 1/GPX1 Antibody (JF0944) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Glutathione Peroxidase 1/GPX1 aa 1-50 / 203. (SwissProt: P07203 Human; SwissProt: P04041 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:1000
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody (JF0944)

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP2-66874)(1)

Publication using NBP2-66874

• Xue C, Dou J, Zhang S et al. Shikonin potentiates skin wound healing in Sprague-Dawley rats by stimulating fibroblast and endothelial cell proliferation and angiogenesis The journal of gene medicine 2023-11-28 [PMID: 38017625]

Bioinformatics

• Gene Symbol: GPX1