GLMN Overexpression Lysate (Native)

Images

 
Western Blot: GLMN Overexpression Lysate (Adult Normal) [NBL1-11116] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GLMN.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GLMN Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201521 and based on accession number NM_053274. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens glomulin, FKBP associated protein (GLMN), mRNA.
Gene
GLMN

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GLMN

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GLMN Overexpression Lysate (Native)

  • FAP
  • FAP48FAP68
  • FKBPAP
  • GLMLFK506-binding protein-associated protein
  • glomulin
  • glomulin, FKBP associated protein
  • GVM
  • GVMFKBP-associated protein
  • VMGLOMvenous malformation with glomus cells

Background

This gene encodes a phosphorylated protein that is a member of a Skp1-Cullin-F-box-like complex. The protein is essential for normal development of the vasculature and mutations in this gene have been associated with glomuvenous malformations, also called glomangiomas. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89649
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF4885
Species: Mu
Applications: WB, IP
AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NB100-621
Species: Hu, Mu
Applications: WB, IHC-P, IP
NB100-56552
Species: Hu
Applications: WB
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB600-1032
Species: Hu, Mu, Rt, Ba
Applications: WB, ICC/IF, IHC
NBP1-84993
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-01521
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBL1-11116
Species: Hu
Applications: WB

Publications for GLMN Lysate (NBL1-11116) (0)

There are no publications for GLMN Lysate (NBL1-11116).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GLMN Lysate (NBL1-11116) (0)

There are no reviews for GLMN Lysate (NBL1-11116). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GLMN Lysate (NBL1-11116) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GLMN Products

Bioinformatics Tool for GLMN Lysate (NBL1-11116)

Discover related pathways, diseases and genes to GLMN Lysate (NBL1-11116). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GLMN Lysate (NBL1-11116)

Discover more about diseases related to GLMN Lysate (NBL1-11116).
 

Pathways for GLMN Lysate (NBL1-11116)

View related products by pathway.

PTMs for GLMN Lysate (NBL1-11116)

Learn more about PTMs related to GLMN Lysate (NBL1-11116).
 

Research Areas for GLMN Lysate (NBL1-11116)

Find related products by research area.

Blogs on GLMN

There are no specific blogs for GLMN, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GLMN Antibody
NBP2-16669

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GLMN Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GLMN