Home » GIT2 » GIT2 Antibody Pairs » GIT2 Antibody Pair

GIT2 Antibody Pair

 Submit a Review

Images

 
There are currently no images for GIT2 Antibody Pair (H00009815-PW1).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP

Order Details

100% Guarantee on every product

GIT2 Antibody Pair Summary

Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: mouse monoclonal anti-GIT2 (300 ug)
  • Antibody pair for WB: rabbit polyclonal anti-GIT2 (50 ul)
    		•
    Gene
    GIT2
    Purity
    Immunogen affinity purified

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    Quality control test: Immunoprecipitation-Western Blot (IP-WB).

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for GIT2 Antibody Pair

    • ARF GAP GIT2
    • ARF GTPase-activating protein GIT2
    • CAT2
    • CAT-2
    • cool-associated, tyrosine phosphorylated protein 2
    • Cool-interacting tyrosine-phosphorylated protein 2
    • DKFZp686G01261
    • G protein-coupled receptor kinase interacting ArfGAP 2
    • G protein-coupled receptor kinase interactor 2
    • G protein-coupled receptor kinase-interactor 2
    • KIAA0148GRK-interacting protein 2
    • MGC760

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

    Customers Who Viewed This Item Also Viewed...

    CRAT Antibody
    NBP1-86616
    Western Blot: CRAT Antibody [NBP1-86616] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
    Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
    Species: Hu
    Applications: WB, IHC, IHC-P
    Catalase Antibody
    NBP2-24916
    Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
    Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
    (1 Review    )
    (4 Publications    )
    Species: Hu, Mu, Rt, Bv
    Applications: WB, IHC, IHC-P
    GIT1 Antibody
    (924640) [Unconjugate
    MAB8508
    GIT1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB8508) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
    Western blot shows lysates of HUVEC human umbilical vein endothelial cells, U2OS human osteosarcoma cell line, M1 mouse myeloid leukemia cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB8508) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GIT1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    SH‑SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB8508, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
    Species: Hu, Mu, Rt
    Applications: WB, IHC, CyTOF-ready, ICFlow
    CAT1 Antibody
    NBP1-90066
    Western Blot: CAT1 Antibody [NBP1-90066] - Analysis in control (vector only transfected HEK293T lysate) and SLC7A1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
    Immunohistochemistry-Paraffin: CAT1 Antibody [NBP1-90066] - Staining of human small intestine shows strong membranous positivity in glandular cells.
    Species: Hu
    Applications: WB, IHC, IHC-P
    TRPV6 Antibody
    NBP1-74138
    Western Blot: TRPV6 Antibody [NBP1-74138] - Sample Type: hRetinal pigment epithelial cells, 4 individual donors (20ug) Primary Dilution: 1:1000 Secondary Antibody: goat anti-rabbit-AP Secondary Dilution: 1:2000 Image Submitted by: Brian Kennedy Indiana University Northwest
    Immunocytochemistry/Immunofluorescence: TRPV6 Antibody [NBP1-74138] - hRetinal pigment epithelial cells Green: primary Red: nuclear Primary Dilution: 1:200 Secondary Antibody: goat anti-rabbit-Alexa 488 Secondary Dilution: 1:500 Image Submitted by: Brian Kennedy Indiana University Northwest
    Western Blot: TRPV6 Antibody [NBP1-74138] - Rat Brain Lysate 1ug/ml Gel Concentration 12%
    Species: Hu, Rt
    Applications: WB, ICC/IF
    iNOS Antibody
    NB300-605
    Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
    Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
    Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Both normal and cancer biopsies of deparaffinized human Lung tissue.
    (2 Reviews    )
    (22 Publications    )
    Species: Hu, Mu, Rt
    Applications: WB, ICC/IF, IHC, IHC-P
    GLYAT Antibody
    (4D1)
    NBP2-45897
    Western Blot: GLYAT Antibody (4D1) [NBP2-45897] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLYAT.
    Immunohistochemistry: GLYAT Antibody (4D1) [NBP2-45897] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
    Immunohistochemistry: GLYAT Antibody (4D1) [NBP2-45897] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
    Species: Hu
    Applications: WB, IHC
    CAT3/SLC7A3
    Antibody
    NBP1-78911
    Western Blot: SLC7A3 Antibody [NBP1-78911] - Western blot analysis of SLC7A3 expression in Myc-tagged SLC7A3 transfected 293T (2, 3) and parental cell (1), using NBP1-78911 (1, 2) or anti-Myc-tag antibody (3).
    Immunocytochemistry/Immunofluorescence: SLC7A3 Antibody [NBP1-78911] - Immunocytochemical detection of SLC7A3 in 293T transiently expressing SLC7A3 with NBP1-78911 (right) or normal rabbit IgG (left).
    Immunohistochemistry-Paraffin: SLC7A3 Antibody [NBP1-78911] - Immunohistochemical detection of SLC7A3 on paraffin embedded section of human thyroid with SLC7A3 Antibody.
    Species: Hu
    Applications: WB, Flow, ICC/IF, IHC, IHC-P
    ISYNA1 Antibody
    NBP2-47415
    Western Blot: ISYNA1 Antibody [NBP2-47415] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293
    Immunocytochemistry/Immunofluorescence: ISYNA1 Antibody [NBP2-47415] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & cytosol.
    Immunohistochemistry-Paraffin: ISYNA1 Antibody [NBP2-47415] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P
    Paxillin Antibody
    [Unconjugated]
    AF4259
    Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
    Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Paxillin at approximately 70 & 69 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
    Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4259) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips and cytosol. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
    Species: Hu, Mu, Rt
    Applications: WB, Simple Western, ICC
    Nanos2 Antibody
    NBP1-76917
    Western Blot: Nanos2 Antibody [NBP1-76917] - Rat brain tissue lysate with Nanos2 antibody at (A) 1 and (B) 2 ug/mL.
    Immunocytochemistry/Immunofluorescence: Nanos2 Antibody [NBP1-76917] - Immunofluorescence of Nanos2 in Human Testis cells with Nanos2 antibody at 20 ug/mL.
    Immunohistochemistry-Paraffin: Nanos2 Antibody [NBP1-76917] - Human brain tissue cells with Nanos2 antibody at 2.5 ug/ml.
    Species: Hu, Mu, Rt
    Applications: WB, ELISA, ICC/IF, IHC, IHC-P
    SLC7A2 Antibody
    NBP2-58924
    Immunocytochemistry/Immunofluorescence: SLC7A2 Antibody [NBP2-58924] - Staining of human cell line MCF7 shows localization to plasma membrane.
    Species: Hu
    Applications: ICC/IF
    nNOS Antibody
    NB100-858
    Western Blot: nNOS Antibody [NB100-858] - Western blot analysis of nNOS in human skeletal muscle extracts (RIPA buffer, 35ug total protein per lane) using NB100-858 at 0.1 ug/ml.
    Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    (1 Review    )
    (8 Publications    )
    Species: Hu, Mu, Rt, GP, Mk
    Applications: WB, ICC/IF, IHC, IHC-Fr, PEP-ELISA, IHC-WhMt
    p19ARF Antibody
    NB200-106
    Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
    Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
    (40 Publications    )
    Species: Mu
    Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
    eNOS Antibody
    NB300-500
    Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
    Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
    (3 Reviews    )
    (7 Publications    )
    Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
    Applications: WB, IHC, IHC-Fr, IHC-P
    FAK Antibody (4D11)
    NBP2-45923
    Western Blot: FAK Antibody (4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK.
    Immunohistochemistry: FAK Antibody (4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
    Immunohistochemistry: FAK Antibody (4D11) [NBP2-45923] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P

    Publications for GIT2 Antibody Pair (H00009815-PW1) (0)

    There are no publications for GIT2 Antibody Pair (H00009815-PW1).
    By submitting your publication information earn gift cards and discounts for future purchases.

    Reviews for GIT2 Antibody Pair (H00009815-PW1) (0)

    There are no reviews for GIT2 Antibody Pair (H00009815-PW1). By submitting a review earn points towards our Rewards Program.
    • 250 points for product review
    • 500 additional points for an image with your product review
    • Double points (500) if you are the first to review this product
    • Double points (1000) if you are the first to review this product with an image

    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

    FAQs for GIT2 Antibody Pair (H00009815-PW1) (0)

    There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

    Additional GIT2 Antibody Pair Products

    Related Products by Gene

    CAT, CATIONIC AMINO ACID TRANSPORTER 1, CDKN2A, CRAT, GIT1, GLYAT, INDUCIBLE NITRIC OXIDE SYNTHASE, ISYNA1, NANOS2, NOS1, NOS2, NOS3, PTK2, PXN, SLC7A1, SLC7A2, SLC7A3, TNF, TNFSF14, TRPV6

    Bioinformatics Tool for GIT2 Antibody Pair (H00009815-PW1)

    Discover related pathways, diseases and genes to GIT2 Antibody Pair (H00009815-PW1). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
    Visit Tool

    Diseases for GIT2 Antibody Pair (H00009815-PW1)

    Discover more about diseases related to GIT2 Antibody Pair (H00009815-PW1).
    		 

    Pathways for GIT2 Antibody Pair (H00009815-PW1)

    View related products by pathway.

    PTMs for GIT2 Antibody Pair (H00009815-PW1)

    Learn more about PTMs related to GIT2 Antibody Pair (H00009815-PW1).

    Blogs on GIT2

    There are no specific blogs for GIT2, but you can read our latest blog posts.

    Contact Information

    Product PDFs

      PDF Datasheet
      Request CofA
      Request SDS

    Review this Product

    Be the first to review our GIT2 Antibody Pair and receive a gift card or discount.


    Bioinformatics

    Gene Symbol GIT2

    Customers Who Bought This Also Bought

    Other Recombinant Human GIT2 Protein
    Recombinant Human GIT2 Protein
    H00009815-Q01-10ug
    Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
    p19ARF Antibody
    NB200-106
    Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
    p19ARF Antibody
    NB200-106
    Immunohistochemistry-Paraffin: GIT2 Antibody [NBP1-19143] - FFPE section of human lung carcinoma. Affinity purified rabbit anti-GIT2 used at a dilution of 1:500.
    GIT2 Antibody
    NBP1-19143
    Immunohistochemistry: GIT2 Antibody [NBP1-19143] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GIT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
    GIT2 Antibody
    NBP1-19143
    Immunoprecipitation: GIT2 Antibody [NBP1-19143] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-19143 used for IP at 3 mcg/mg lysate. GIT2 was also immunoprecipitated by NBP1-19144 which recognize downstream epitopes.
    GIT2 Antibody
    NBP1-19143
    Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
    iNOS Antibody
    NB300-605
    Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
    iNOS Antibody
    NB300-605
    Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Both normal and cancer biopsies of deparaffinized human Lung tissue.
    iNOS Antibody
    NB300-605