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GIT2 Antibody Pair

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal

Order Details

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GIT2 Antibody Pair Summary

Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.

  • Antibody pair for IP: mouse monoclonal anti-GIT2 (300 ug)
  • Antibody pair for WB: rabbit polyclonal anti-GIT2 (50 ul)
    		•
    Clonality
    Polyclonal
    Gene
    GIT2
    Purity
    Immunogen affinity purified

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    Quality control test: Immunoprecipitation-Western Blot (IP-WB).

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for GIT2 Antibody Pair

    • ARF GAP GIT2
    • ARF GTPase-activating protein GIT2
    • CAT2
    • CAT-2
    • cool-associated, tyrosine phosphorylated protein 2
    • Cool-interacting tyrosine-phosphorylated protein 2
    • DKFZp686G01261
    • G protein-coupled receptor kinase interacting ArfGAP 2
    • G protein-coupled receptor kinase interactor 2
    • G protein-coupled receptor kinase-interactor 2
    • KIAA0148GRK-interacting protein 2
    • MGC760

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86615] - Staining of human tonsil shows low expression as expected.
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    Catalase Antibody
    NBP2-24916
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    GIT1 Antibody
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    GIT1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB8508) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
    Western blot shows lysates of HUVEC human umbilical vein endothelial cells, U2OS human osteosarcoma cell line, M1 mouse myeloid leukemia cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB8508) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GIT1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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    CAT1 Antibody
    NBP1-90066
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    TRPV6 Antibody
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    Western Blot: TRPV6 Antibody [NBP1-74138] - Sample Type: hRetinal pigment epithelial cells, 4 individual donors (20ug) Primary Dilution: 1:1000 Secondary Antibody: goat anti-rabbit-AP Secondary Dilution: 1:2000 Image Submitted by: Brian Kennedy Indiana University Northwest
    Immunocytochemistry/Immunofluorescence: TRPV6 Antibody [NBP1-74138] - hRetinal pigment epithelial cells Green: primary Red: nuclear Primary Dilution: 1:200 Secondary Antibody: goat anti-rabbit-Alexa 488 Secondary Dilution: 1:500 Image Submitted by: Brian Kennedy Indiana University Northwest
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    GLYAT Antibody
    NBP2-55298
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    CAT3/SLC7A3
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    ISYNA1 Antibody
    NBP2-47415
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    Paxillin Antibody
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    AF4259
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    Nanos2 Antibody
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    SLC7A2 Antibody
    NBP2-58924
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    TNF-alpha
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    210-TA/CF
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    nNOS Antibody
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    Western Blot: nNOS Antibody [NB100-858] - Staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
    Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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    p19ARF Antibody
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    Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
    Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
    Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
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    LIGHT/TNFSF14
    [Unconjugated]
    664-LI/CF
    1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
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    eNOS Antibody
    NB300-500
    Immunohistochemistry: eNOS Antibody [NB300-500] - The pulmonary tissues were analyzed for expression of eNOS protein by immunohistochemistry(IHC). Dark brown staining of indicates eNOS-positive cells. IHC stain of representative pulmonary artery endothelial cells from rats subjected to CON=Control Group, ALI=Acute lung injury Group, LV=Low tidal volume Group, VLV=Very low tidal volume Group, MV=Large tidal volume Group (magnification, x400).
    Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Staining of eNOS in human lung tissue.
    Immunohistochemistry-Paraffin: eNOS Antibody [NB300-500] - Mouse Carotid Artery stained with eNOS antibody. Image from verified customer review.
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    FAK Antibody
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    Western Blot: FAK Antibody (4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK.
    Immunocytochemistry/Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923] - FAK Antibody (4D11) [NBP2-45923] - Analysis of HeLa cells.
    Immunohistochemistry: FAK Antibody (4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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    Bioinformatics

    Gene Symbol GIT2