Recombinant Human GIP His Protein

SDS-Page: Recombinant Human GIP Protein [NBP2-23070]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human GIP His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 22-153 of Human His-tag GIP
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEKKEGHF SALPSLPVGS HAKVSSPQPR GPRYAEGTFI SDYSIAMDKI HQQDFVNWLL AQKGKKNDWK HNITQREARA LELAGQANRK EEEAVEPQSS PAKNPSDEDL LRDLLIQELL ACLLDQTNLC RLRSR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GIP
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 50% glycerol, 0.1 M NaCl, 2 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human GIP His Protein

• gastric inhibitory polypeptide
• GIP
• Glucose-dependent Insulinotropic Polypeptide
• Incretin

Background

GIP, also known as glucose-dependent insulinotropic polypeptide, is an important insulin-releasing hormone of the enteroinsular axis that has a functional profile of possible therapeutic value for type 2 diabetes. This protein is an important incretin hormone released into the circulation from endocrine K-cells of the duodenum and jejunum after ingestion of food1. It was evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. It also promotes plasma triglyceride clearance in response to oral fat loading. In liver, GIP has been shown to enhance insulin-dependent inhibition of glycogenolysis. Recombinant human GIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GIP