GGCX Antibody

Western Blot: GGCX Antibody [NBP2-16651] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 7. 5% SDS PAGE gel, diluted at 1:3000.

Immunocytochemistry/Immunofluorescence: GGCX Antibody [NBP2-16651] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using GGCX antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: GGCX Antibody [NBP2-16651] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Product Discontinued

GGCX Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GGCX. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Predicted Species: Mouse (95%), Rat (94%), Sheep (96%), Rhesus Macaque (99%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GGCX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:30981116).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for GGCX Antibody

• EC 4.1.1.90
• gamma-glutamyl carboxylaseFLJ26629
• GC
• GGCX
• Peptidyl-glutamate 4-carboxylase
• Vitamin K gamma glutamyl carboxylase
• vitamin K-dependent gamma-carboxylase
• VKCFD1

Background

This gene encodes an enzyme which catalyzes the posttranslational modification of vitamin K-dependent protein. Many of these vitamin K-dependent proteins are involved in coagulation so the function of the encoded enzyme is essential for hemostasis. Mutations in this gene are associated with vitamin K-dependent coagulation defect and PXE-like disorder with multiple coagulation factor deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GGCX Antibody (NBP2-16651)(1)

Publication using NBP2-16651

• Ma H, Zhang B L et al. Vitamin K2-Dependent GGCX and MGP Are Required for Homeostatic Calcium Regulation of Sperm Maturation. iScience 2019-04-26 [PMID: 30981116] (IF/IHC, WB, Rat)

Bioinformatics

• Gene Symbol: GGCX
• Uniprot:
  • P38435(Human)