GFR alpha-like Antibody (2345C) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human GFRa-like (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Rabbit Anti-Human GFRa-like Monoclonal Antibody (Catalog # MAB9697) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 2345C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GFR alpha-like Antibody (2345C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human GFR alpha ‑like
  Ser19-Glu351
  Accession # Q6UXV0
• Specificity: Detects human GFR alpha ‑like in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-like Antibody (2345C) [Unconjugated]

• bA360D14.1
• C6orf144
• GDNF family receptor alpha-like
• GFR alpha like
• GFR alpha-like
• GFRAL
• GRAL
• IVFI9356
• UNQ9356

Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71%  identity with mouse and rat GFRAL respectively. It is expressed on both fetal and adult hindbrain neurons of the CNS (3), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling (2-4). GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance (2-4).

1. Li, Z. et al. (2005) J. Neurochem. 95:361.
2. Mullican, S. et al. (2017) Nat. Med 23:1150.
3. Yang, L. et al. (2017) Nat. Med 23: 1158.
4. Emmerson, P. et al (2017) Nat. Med 23:1215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6UXV0()