GCAP3 Antibody (3F10) - Azide and BSA Free

Sandwich ELISA: GCAP3 Antibody (3F10) [H00009626-M03] - Detection limit for recombinant GST tagged GUCA1C is 0.3 ng/ml as a capture antibody.

Immunoprecipitation: GCAP3 Antibody (3F10) [H00009626-M03] - Analysis of GUCA1C transfected lysate using anti-GUCA1C monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GUCA1C rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 3F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GCAP3 Antibody (3F10) - Azide and BSA Free Summary

• Immunogen: GUCA1C (NP_005450.2, 110 a.a. ~ 209 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KNKLLDMFMAVQALNGQQTLSPEEFINLVFHKIDINNDGELTLEEFINGMAKDQDLLEIVYKSFDFSNVLRVICNGKQPDMETDSSKSPDKAGLGKVKMK
• Specificity: GUCA1C - guanylate cyclase activator 1C (3F10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GUCA1C
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GCAP3 Antibody (3F10) - Azide and BSA Free

• GCAP3GCAP 3
• guanylate cyclase activator 1CMGC120159
• guanylyl cyclase-activating protein 3
• MGC120158

Background

GCAP3, also known as GUCA1C or Guanylyl cyclase-activating protein 3, has a 209 amino acid isoform that is 24 kDa and a short 196 amino acid isoform that is approx. 22 kDa; commonly found in retina; in conditions of low free calcium ions concentration stimulates guanylyl cyclase 1 (GC1) and GC2 and when the concentration is elevated inhibits guanylyl cyclases, which is very important for the recuperation of the dark state of rod photoreceptors following light exposure. Disease research has linked this protein with cone dystrophy, neuronitis, and retinitis. This protein has shown interactions with GUCA1A, GUCA1B, GUCY2D, GUCY2F, and CNGA3 proteins in the pathways such as the visual cycle in retinal rods, olfactory transduction, and phototransduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GUCA1C
• Entrez:
  • 9626(Human)
• Uniprot:
  • NP_005450(Human)