GASP-2/WFIKKN Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

GASP-2/WFIKKN Antibody [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GASP‑2/WFIKKN
  Ala20-Asp548
  Accession # Q96NZ8
• Specificity: Detects human GASP‑2/WFIKKN in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human GASP-1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GASP-2/WFIKKN Antibody [Alexa Fluor® 532]

• C16orf12
• Chromosome 16 Open Reading Frame 12
• GASP2
• GASP-2
• Growth And Differentiation Factor-Associated Serum Protein 2
• hGASP-2
• RJD2
• WAP Four-Disulfide Core Domain 20A
• WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1
• WAP, FS, Ig, KU, And NTR-Containing Protein
• WFDC20A
• WFIKKN
• WFIKKN1

Background

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). Tests at R&D Systems have measured the ability of GASP-2 to inhibit trypsin cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (R&D Systems Catalog # ES002). The IC₅₀ value was approximately 10 nM, as measured in a reaction mixture containing 1.0 nM trypsin, 10 µM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.