GAS41 Overexpression Lysate (Native)

Images

 
Western Blot: GAS41 Overexpression Lysate (Adult Normal) [NBL1-17927] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GAS41.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GAS41 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201526 and based on accession number NM_006530. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens YEATS domain containing 4 (YEATS4), mRNA.
Gene
YEATS4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GAS41

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GAS41 Overexpression Lysate (Native)

  • B230215M10Rik
  • Gas41,4930573H17Rik
  • GAS41glioma-amplified sequence-41
  • Glioma-amplified sequence 41
  • nuBI1
  • NUBI-1
  • NuMA binding protein 1
  • NuMA-binding protein 1
  • YAF9
  • YEATS domain containing 4
  • YEATS domain-containing protein 4

Background

The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for GAS41 Lysate (NBL1-17927) (0)

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Bioinformatics

Gene Symbol YEATS4