gamma Tubulin Antibody (JF0972)

Western Blot: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Western blot analysis of gamma Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Staining gamma Tubulin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Analysis of paraffin-embedded mouse testis tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.

Flow Cytometry: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Flow cytometric analysis of gamma Tubulin was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Western Blot: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Staining gamma Tubulin in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Analysis of paraffin-embedded mouse colon tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.

Flow Cytometry: gamma Tubulin Antibody (JF0972) [NBP2-66860] - Analysis of Jurkat cells with gamma Tubulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Discontinued

gamma Tubulin Antibody (JF0972) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human gamma Tubulin aa 227-407 / 451. (SwissProt: P23258 Human; SwissProt: Q9NRH3 Human; SwissProt: P83887 Mouse; SwissProt: Q8VCK3 Mouse; SwissProt: P83888 Rat; SwissProt: P23330 XenopusLaevis)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for gamma Tubulin Antibody (JF0972)

• gamma Tubulin
• Gamma-1-tubulin
• Gamma-tubulin complex component 1
• GCP-1
• TUBG
• TUBG1
• TUBGCP1
• tubulin gamma-1 chain
• tubulin, gamma 1
• tubulin, gamma polypeptide

Background

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBG1