gamma Tubulin Antibody (4D11)

Images

 
Western Blot: gamma Tubulin Antibody (4D11) [NB100-74421] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody ...read more
Immunocytochemistry/ Immunofluorescence: gamma Tubulin Antibody (4D11) [NB100-74421] - Analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (shown in green) in HeLa (A) and U2-OS cells (B).
Immunohistochemistry-Paraffin: gamma Tubulin Antibody (4D11) [NB100-74421] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.
Immunohistochemistry-Paraffin: gamma Tubulin Antibody (4D11) [NB100-74421] - Both normal and cancer biopsies of deparaffinized human Colon tissue.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
4D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

gamma Tubulin Antibody (4D11) Summary

Immunogen
Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.
Marker
Centrosome Marker
Specificity
gamma Tubulin (4D11)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TUBG1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for gamma Tubulin Antibody (4D11)

  • gamma-1-tubulin
  • Gamma-tubulin complex component 1
  • GCP-1
  • TUBG
  • TUBGCP1
  • tubulin gamma-1 chain
  • tubulin, gamma 1
  • tubulin, gamma polypeptide

Background

Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TUBG1
Entrez
Uniprot