FXYD3 Antibody (OTI4E3) - Azide and BSA Free

Western Blot: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXYD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXYD3.

Immunocytochemistry/Immunofluorescence: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FXYD3.

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Human liver tissue using anti-FXYD3 mouse monoclonal antibody.

Western Blot: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-FXYD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FXYD3 mouse monoclonal antibody.

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FXYD3 mouse monoclonal antibody.

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Human bladder tissue using anti-FXYD3 mouse monoclonal antibody.

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Human colon tissue using anti-FXYD3 mouse monoclonal antibody.

Immunohistochemistry: FXYD3 Antibody (OTI4E3) - Azide and BSA Free [NBP2-71219] - Staining of paraffin-embedded Human prostate tissue using anti-FXYD3 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: OTI4E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FXYD3 Antibody (OTI4E3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human FXYD3(NP_005962) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FXYD3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 7.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for FXYD3 Antibody (OTI4E3) - Azide and BSA Free

• Chloride conductance inducer protein Mat-8
• FXYD domain containing ion transport regulator 3
• FXYD domain-containing ion transport regulator 3
• Mammary tumor 8 kDa protein
• MAT8MAT-8
• phospholemman-like protein
• Phospholemman-like
• PLMLMGC111076

Background

FXYD3, also known as FXYD domain-containing ion transport regulator 3, consists of 3 isoforms, a 87 amino acid isoform that is 9 kDa, a 113 amino acid isoform that is 12 kDa, and a 144 amino acid that is 15 kDa; is membrane located, functions as an inducer of a hyperpolarization-activated chloride current when expressed in Xenopus oocytes, and may be a modulator capable of activating endogenous oocyte channels. Current research is being performed on several diseases and disorders including adenocarcinoma, pancreatic, ductal adenocarcinoma, intrahepatic cholangiocarcinoma, colorectal cancer, cholangiocarcinoma prostate carcinoma, melanoma, pancreatic cancer, pancreatitis, prostate cancer, breast cancer, and prostatitis. The FXYD3 protein has also shown an interaction with SERBP1, FBXL12, NR4A1, MAPK6 and NUDT3, in the regulation of catalytic activity, ion transport, and chloride transport pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FXYD3