FNIP1 Antibody - BSA Free

Western Blot: FNIP1 Antibody [NBP3-18249] - Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody (NBP3-18249). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (NBP3-18249) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa.

Immunocytochemistry/Immunofluorescence: FNIP1 Antibody [NBP3-18249] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody (NBP3-18249). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (NBP3-18249) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite.

Immunohistochemistry: FNIP1 Antibody [NBP3-18249] - Immunohistochemistry analysis using Rabbit Anti-FNP1 Polyclonal Antibody (NBP3-18249). Tissue: Renal Cell Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FNP1 Polyclonal Antibody (NBP3-18249) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Immunohistochemistry: FNIP1 Antibody [NBP3-18249] - Immunohistochemistry analysis using Rabbit Anti-FNP1 Polyclonal Antibody (NBP3-18249). Tissue: Renal Cell Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FNP1 Polyclonal Antibody (NBP3-18249) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Product Discontinued

FNIP1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide from the mid-protein of Human FNIP1
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FNIP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Western Blot 1:1000
• Application Notes: Optimal dilutions for assays should be determined by the user.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FNIP1 Antibody - BSA Free

• DKFZp781P0215
• folliculin interacting protein 1
• folliculin-interacting protein 1
• KIAA1961DKFZp686E18167
• MGC667

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FNIP1