Recombinant Human FKBP25 Protein

SDS-Page: Recombinant Human FKBP25 Protein [NBP1-41160] - 25.1 kDa (224aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human FKBP25 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-224 of Human FKBP25
  
  

  Source: E.coli

  Amino Acid Sequence: MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID

• Details of Functionality: Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FKBP3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 25.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human FKBP25 Protein

• EC 5.2.1.8,25 kDa FK506-binding protein
• FK506 binding protein 3, 25kDa
• FK506-binding protein 3 (25kD)
• FK506-binding protein 3
• FKBP25
• FKBP25,25 kDa FKBP
• FKBP3
• FKBP-3
• Immunophilin FKBP25
• peptidyl-prolyl cis-trans isomerase FKBP3
• PPIase FKBP3
• PPIase
• rapamycin binding protein
• Rapamycin-selective 25 kDa immunophilin
• rotamase
• T-cell

Background

FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FKBP3