FKBP12 Antibody (422513) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 422513
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

FKBP12 Antibody (422513) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human FKBP12
  Gly2-Glu108
  Accession # P62942
• Specificity: Detects human, mouse, and rat FKBP12 in Western blots. In Western blots, no cross-reactivity with other FKBP family members is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FKBP12 Antibody (422513) [Alexa Fluor® 750]

• 12 kDa FKBP
• EC 5.2.1.8
• FK506 binding protein 1A, 12kDa
• FK506-binding protein 1A
• FKBP1
• FKBP12
• FKBP12C
• FKBP12FKBP12-Exip3
• FKBP-12T-cell, 12-kD
• FKBP1a
• FKBP-1A
• peptidyl-prolyl cis-trans isomerase FKBP1A
• PKC12
• PPIase FKBP1A
• PPIASE
• protein kinase C inhibitor 2
• rotamase

Background

FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. Proline isomerase activity was demonstrated but not used for quality control. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity (2) and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.