Filensin Antibody (FIL-7B10)

Product Discontinued

Filensin Antibody (FIL-7B10) Summary

• Immunogen: Human and bovine lens filament enriched fraction (plasma membrane cytoskeleton complex).
• Specificity: The antibody recognizes an epitope located within either the rod or the N-terminal non-(a) helical domain of the filensin molecule. May be used in immunoblotting where it specifically detects both the full-length filensin (115 kDa), the processed fragment, and a variety of breakdown products in the mol. wt. range of 28-69 kDa. Does not react with cultured lens epithelial cells nor with normal human skin, psoriatic or tumoral skin (basal cell carcinoma).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BFSP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 115 kDa (full length filensin) and a band of approximately 53 kDa (processed filensin fragment). It also reacts with a variety of breakdown products of filensin in the range of 28-69 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Cow and Sheep.Does not cross-react with Mouse, Rat or Chicken.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Filensin Antibody (FIL-7B10)

• Beaded filament structural protein 1
• beaded filament structural protein 1, filensin
• CP115Lens intermediate filament-like heavy
• filensin
• Lens fiber cell beaded-filament structural protein CP 115
• LIFL-H115 KD

Background

More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, CP49 (also known as phakinin) and filensin (or CP115), are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BFSP1
• Entrez:
  • 631(Human)
  • 12075(Mouse)
  • 25394(Rat)