Fibroblast Activation Protein alpha/FAP Antibody

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NBP2-89135] - Analysis of FAP on Siha cell lysate using anti-FAP antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Fibroblast Activation Protein alpha/FAP Antibody [NBP2-89135] - Staining FAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NBP2-89135] - Analysis of SH-SY5Y cells with FAP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Fibroblast Activation Protein alpha/FAP Antibody [NBP2-89135] - Staining FAP in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Fibroblast Activation Protein alpha/FAP Antibody Summary

• Immunogen: Synthetic peptide within human Fibroblast Activation Protein alpha/FAP aa 715-757. (SwissProt: Q12884 Human;SwissProt: P97321 Mouse)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAP
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.5% BSA, 50% Glycerol.
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Activation Protein alpha/FAP Antibody (NBP2-89135)(1)

Publication using NBP2-89135

• Wu J, Jing X, Du Q et al. Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI-1-TGF-?-myoCAF-Dependent Mechanism Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-06-17 [PMID: 37330661] (WB, Mouse)
  
  Details:
  IHC-P: antibdy dilution: 1:200. Antigen retrieval: citrate-based solution pH 6.0 (H-3300, Vector)

Bioinformatics

• Gene Symbol: FAP