Fibroblast Activation Protein alpha/FAP Antibody

Product Discontinued

Fibroblast Activation Protein alpha/FAP Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human FAP-1. The immunogen region is: Internal.
• Specificity: FAP-1 Antibody detects endogenous levels of total FAP-1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fibroblast Activation Protein alpha/FAP Antibody (NBP1-67557)(2)

Publications using NBP1-67557

• Xia Q, Zhang FF, Geng F et al. Improvement of anti-tumor immunity of fibroblast activation protein alpha based vaccines by combination with cyclophosphamide in a murine model of breast cancer. Cell. Immunol. 2016-08-16 [PMID: 27545090] (WB)
• Xia Q, Zhang FF, Geng F et al. Anti-tumor effects of DNA vaccine targeting human fibroblast activation protein alpha by producing specific immune responses and altering tumor microenvironment in the 4T1 murine breast cancer model. Cancer Immunol. Immunother. 2016-05-01 [PMID: 27020681] (WB, Human)

FAQs for Fibroblast Activation Protein alpha/FAP Antibody (NBP1-67557). (Showing 1 - 1 of 1 FAQs).

1. Is this antibody approved for use with an IHC method that involves paraffin imbedded samples? What species and tissue sample was this antibody tested on using the IHC method?
   • NBP1-67557 was tested on paraffin-embedded human breast carcinoma tissue, and found to give positive results.

Bioinformatics

• Gene Symbol: FAP
• Entrez:
  • 2191(Human)
• OMIM:
  • 600403(Human)
• Uniprot:
  • Q12884()