Fibroblast Activation Protein alpha/FAP Antibody (427819) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 427819
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

Fibroblast Activation Protein alpha/FAP Antibody (427819) [Alexa Fluor™ Plus 594] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
• Specificity: Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 and DPP4 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody (427819) [Alexa Fluor™ Plus 594]

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha ₃ beta ₁, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.