FGF-17 Antibody

Recombinant Human FGF‑17 (Catalog # 319-FG) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF319). The ND50 is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF-17 Antibody Summary

• Immunogen: E. coli-derived recombinant human FGF-17
  Thr23-Thr216
  Accession # O60258
• Specificity: Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-10, and rhFGF-18 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF17
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 6-24 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-17 Antibody

• FGF-13
• FGF17
• FGF-17
• fibroblast growth factor 17

Background

Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30-70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF-17 shares 98.6% amino acid (aa) sequence identity with mouse FGF-17. Rat FGF-17 shares 100% aa sequence identity with mouse FGF-17.

1. Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
2. Xu, J. et al. (1999) Mech. Dev. 83:165.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF17
• Uniprot:
  • O60258()