Recombinant Human FGF-16 Protein

SDS-Page: Recombinant Human FGF-16 Protein [NBP2-61311]

• Reactivity: Hu
• Applications: WB, Bioactivity

Recombinant Human FGF-16 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 206 amino acids corresponding to FGF-16 Source: E. coli

  Uniprot ID: O43320

  Amino Acid Sequence: AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR

• Details of Functionality: FGF-16 protein is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF16
• Purity: >98%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of FGF-16 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Western Blot
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in 20 mM Tris-HCl, 1 M NaCl, pH 9.0, 0.02 % Tween-20, 10% Glycerol.
• Purity: >98%, by SDS-PAGE and HPLC

Alternate Names for Recombinant Human FGF-16 Protein

• FGF16
• FGF-16
• fibroblast growth factor 16

Background

The protein encoded by the FGF16 gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The rat homolog is predominantly expressed in embryonic brown adipose tissue and has significant mitogenic activity, which suggests a role in proliferation of embryonic brown adipose tissue. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: FGF16