FFAR4/GPR120 Antibody

Images

 
Western Blot: GPR120 Antibody [NBP1-70975] - Analysis of extracts from LOVO cells, using GPR120 Antibody. The lane on the right is treated with the synthesized peptide.
Immunocytochemistry/ Immunofluorescence: GPR120 Antibody [NBP1-70975] - Analysis of LOVO cells, using GPR120 Antibody. The picture on the right is treated with the synthesized peptide.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FFAR4/GPR120 Antibody Summary

Immunogen
The antiserum was produced against synthesized peptide derived from human GPR120. The immunogen region is: Internal.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FFAR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FFAR4/GPR120 Antibody

  • FFAR4
  • G protein-coupled receptor 120
  • G protein-coupled receptor 129
  • G protein-coupled receptor PGR4
  • GPR120
  • GPR129
  • G-protein coupled receptor 120
  • G-protein coupled receptor 129
  • G-protein coupled receptor GT01
  • G-protein coupled receptor PGR4
  • G-protein-coupled receptor GT01
  • GT01
  • MGC119984
  • O3FAR1
  • omega-3 fatty acid receptor 1
  • PGR4
  • PGR4DKFZp686F0824

Background

GPR120 is a member of the rhodopsin family of G protein-coupled receptors (GPRs), which was found in 2003 by Fredriksson et al. GPR120 coded by 377 a.a with 7 transmembrane domain, and ERM motif, hightly expressed in stomach, lung, colon, rectum and ileum. GPR120 regulated calcium mobilization, MAP kinase pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FFAR4/GPR120 Antibody (NBP1-70975). (Showing 1 - 3 of 3 FAQ).

  1. I am interested in one of your antibodies, The cat number is NBP1-70975. It said the Ab can be used for IF, can I used for FACS?
    • We haven't tested it for FACS, therefore we can't guarantee it will work. But, if you want to purchase it to test in FACS, you will qualify for our Innovators Reward Program. Our Innovator's Reward program is designed to support your innovative research with minimal financial risk to you. Should you decide to use one of our products in an application or species for which it has not been tested.
  2. It is stated in the datasheet that this antibody has been raised against the internal peptide, would you please provide the sequence of the peptide? I would like to know if this peptide is against the cytoplasmic domain or the membranous domain.
    • The amino acid range of this immunogen is aa 232-282 of human omega-3 fatty acid receptor 1 with ACC# Q5NUL3. This antibody binds to the third cytoplasmic domain of human omega-3 fatty acid receptor 1.
  3. I noticed that this Ab can be used for IF, how did you fix and permerblize the cells? And can I use it for mouse GPR120?
    • NBP1-70975 has been tested and validated to work in mouse samples. Here is our recommendation for IF fixation and permeabolization, although you may need to optimize this based on your samples: 1. Pull off culture medium and add 10% formalin to each well. Let sit at room temperature for 10 minutes. 2. Take off the formalin and permeabilize cells as follows, depending on protein type: a. For detection of either nuclear or mitochondrial protein, add 1 ml TBS with 0.5% Triton X-100. Incubate for 10 minutes at room temperature. b.For detection of a cytoplasmic or membrane protein, add 1 ml PBS/ with 0.5% Tween-20 for 10 minutes at room temperature.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FFAR4
Entrez
OMIM
Uniprot