FFAR1/GPR40 Antibody

Product Discontinued

FFAR1/GPR40 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human GPR40.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FFAR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FFAR1/GPR40 Antibody

• FFA1
• FFAR1
• free fatty acid receptor 1
• G protein-coupled receptor 40
• GPCR40
• GPR40
• GPR40FFA1R
• G-protein coupled receptor 40

Background

G protein coupled receptors provide attractive targets for drug therapy due to the sheer size and diversity of ligands within this receptor family. G proteincoupled receptor 40 (GPR40) functions as a cell-surface receptor for longchain free fatty acids (FFAs). FFAs provide an important energy source, but also function as signaling molecules in various pathways, notably the process of insulin secretion. In pancreatic tissue, the interaction of long chain FFAs with GPR40 amplifies glucose-stimulated insulin secretion from beta cells, suggesting a possible role for GPR40 in the treatment of diabetes associated with insulin-deficiency. Specifically, the Arg211His polymorphism in the GPR40 gene may contribute to the variation of insulin secretory capacity in Japanese men. Also, GPR40 may be involved in the control of breast cancer cell growth by fatty acids and, therefore, provide a link between fat and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FFAR1/GPR40 Antibody (NBP1-00860)(2)

Publications using NBP1-00860

• Wang X, England A, Sinclair C et al. Trans-11 vaccenic acid improves glucose homeostasis in a model of type 2 diabetes by promoting insulin secretion via GPR40 Journal of Functional Foods 2019-09-01 (WB, Rat, Human)
• Lin C, Chao H, Li Z et al. Omega-3 fatty acids regulate NLRP3 inflammasome activation and prevent behavior deficits after traumatic brain injury Exp Neurol. 2017-04-01 [PMID: 28077338] (WB, Rat, Human)

Bioinformatics

• Gene Symbol: FFAR1
• Entrez:
  • 2864(Human)
  • 233081(Mouse)
  • 266607(Rat)
• Uniprot:
  • O14842(Human)