Ferroportin/SLC40A1 Antibody (8G10NB) [FITC]

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Flow Cytometry: Ferroportin/SLC40A1 Antibody (8G10NB) [FITC] [NBP2-45356F] - An intracellular stain was performed on HepG cells with Ferroportin/SLC40A1 Antibody (8G10NB) [FITC] (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
8G10NB
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

Ferroportin/SLC40A1 Antibody (8G10NB) [FITC] Summary

Immunogen
This Ferroportin/SLC40A1 Antibody (8G10NB) was developed against partial recombinant human Ferroportin protein (between amino acids 150-350) [Uniprot: Q9NP59].
Localization
Membrane, Cytoplasm
Predicted Species
Mouse (94%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SLC40A1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Ferroportin/SLC40A1 Antibody (8G10NB) [FITC]

  • Ferroportin
  • Ferroportin-1
  • FPN1
  • FPN1IREG1ferroportin 1
  • HFE4
  • HFE4ferroportin-1
  • IREG1
  • iron regulated gene 1
  • Iron-regulated transporter 1
  • member 3
  • MST079
  • MSTP079
  • MTP1
  • putative ferroportin 1 variant IIIB
  • SLC11A3
  • SLC11A3iron regulated gene 1
  • SLC40A1
  • solute carrier family 11 (proton-coupled divalent metal ion transporters)
  • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
  • solute carrier family 40 (iron-regulated transporter), member 1
  • solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-45356AF350
Alexa Fluor 405 NBP2-45356AF405
Alexa Fluor 488 NBP2-45356AF488
Alexa Fluor 532 NBP2-45356AF532
Alexa Fluor 594 NBP2-45356AF594
Alexa Fluor 647 NBP2-45356AF647
Alexa Fluor 700 NBP2-45356AF700
Alexa Fluor 750 NBP2-45356AF750
Allophycocyanin NBP2-45356APC
Allophycocyanin/Cy7 NBP2-45356APCCY7
Biotin NBP2-45356B
DyLight 350 NBP2-45356UV
DyLight 405 NBP2-45356V
DyLight 488 NBP2-45356G
DyLight 550 NBP2-45356R
DyLight 594 NBP2-45356DL594
DyLight 650 NBP2-45356C
DyLight 680 NBP2-45356FR
DyLight 755 NBP2-45356IR
FITC NBP2-45356F
HRP NBP2-45356H
Janelia Fluor 549 NBP2-45356JF549
Janelia Fluor 646 NBP2-45356JF646
PE NBP2-45356PE
PE/Atto594 NBP2-45356PEATT594
PE/Cy5.5 NBP2-45356PECY55
PE/Cy7 NBP2-45356PECY7
PerCP NBP2-45356PCP

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Bioinformatics

Gene Symbol SLC40A1