FENS1 Antibody

Immunohistochemistry-Paraffin: FENS1 Antibody [NB300-921] - (4ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FENS1 Antibody Summary

• Description: Novus Biologicals Goat FENS1 Antibody (NB300-921) is a polyclonal antibody validated for use in IHC and ELISA. Anti-FENS1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence VVGCSLATGFSPH corresponding to C-Terminus according to NP_065881.1.
• Marker: Early Endosome Marker
• Specificity: This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127)
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WDFY1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave no signal but low background in human liver and HepG2 lysates at up to 1 ug/ml. IHC-P: Human liver shows vesicular staining in the cytoplasm of hepatoicytes.
• Control:

  FENS1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FENS1 Antibody

• FENS-1Zinc finger FYVE domain-containing protein 17
• KIAA1435
• Phosphoinositide-binding protein 1
• WD repeat and FYVE domain containing 1
• WD repeat and FYVE domain-containing protein 1
• WD40 and FYVE domain containing 1
• WDF1WD40- and FYVE domain-containing protein 1
• ZFYVE17phosphoinositide-binding protein SR1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FENS1 Antibody (NB300-921)(1)

Publication using NB300-921

• Ridley SH, Ktistakis N, Davidson K et al. FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments. J Cell Sci 2001-11-01 [PMID: 11739631]

Bioinformatics

• Gene Symbol: WDFY1
• Entrez:
  • 57590(Human)
  • 69368(Mouse)
  • 301549(Rat)
• Uniprot:
  • NP_065881(Human)