FBXW2 Antibody

Images

 
Western Blot: FBXW2 Antibody [NB100-1300] - (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry: FBXW2 Antibody [NB100-1300] - (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FBXW2 Antibody Summary

Immunogen
Peptide with sequence C-KRGSSFLAGEHPG corresponding to C-Terminus according to NP_036296.2.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FBXW2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1.5 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human kidney lysates (calculated MW of 48.0 kDa band according to NP_036296.1). A stronger band of unknown identity was also consistently observed at approx. 30 kDa band - both bands were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney show cytoplasmic staining of epithelial cells in some renal tubules. Anonymous customers have reported positive results in on mouse and rat kidney tissue.
Control
FBXW2 Overexpression Lysate
Publications
Read Publication using NB100-1300.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FBXW2 Antibody

  • F-box and WD repeat domain containing 2
  • F-box and WD-40 domain protein 2
  • F-box and WD-40 domain-containing protein 2
  • F-box/WD repeat-containing protein 2
  • FBW2Fwd2
  • FWD2
  • Md6
  • MGC117371
  • Protein MD6

Background

F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. A large family of mammalian F-box proteins has recently been identified and classified into three groups based on the presence of either the WD-40 repeats, the leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. The FBXW2 gene product, the second identified member of the F-box gene family, contains multiple WD-40 repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FBXW2 Antibody (NB100-1300)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-1300 Applications Species
Winston JT, Koepp DM, Zhu C et al. A family of mammalian F-box proteins. Curr Biol 1999 Oct 21 [PMID: 10531037]

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FBXW2
Entrez
Uniprot