FBXW2 Antibody

Western Blot: FBXW2 Antibody [NB100-1300] - (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry: FBXW2 Antibody [NB100-1300] - (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FBXW2 Antibody Summary

• Immunogen: Peptide with sequence C-KRGSSFLAGEHPG corresponding to C-Terminus according to NP_036296.2.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FBXW2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1.5 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human kidney lysates (calculated MW of 48.0 kDa band according to NP_036296.1). A stronger band of unknown identity was also consistently observed at approx. 30 kDa band - both bands were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney show cytoplasmic staining of epithelial cells in some renal tubules. Anonymous customers have reported positive results in on mouse and rat kidney tissue.
• Control:

  FBXW2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXW2 Antibody

• F-box and WD repeat domain containing 2
• F-box and WD-40 domain protein 2
• F-box and WD-40 domain-containing protein 2
• F-box/WD repeat-containing protein 2
• FBW2Fwd2
• FWD2
• Md6
• MGC117371
• Protein MD6

Background

F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. A large family of mammalian F-box proteins has recently been identified and classified into three groups based on the presence of either the WD-40 repeats, the leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. The FBXW2 gene product, the second identified member of the F-box gene family, contains multiple WD-40 repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXW2 Antibody (NB100-1300)(1)

Publication using NB100-1300

• Winston JT, Koepp DM, Zhu C et al. A family of mammalian F-box proteins. Curr Biol 1999 Oct 21 [PMID: 10531037]

Bioinformatics

• Gene Symbol: FBXW2
• Entrez:
  • 26190(Human)
• Uniprot:
  • NP_036296.(Human)