FBXL3 Antibody

Western Blot: FBXL3 Antibody [NB100-53784] - Analysis of FBXL3 in human Adipose lysate using NB100-53784. Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: FBXL3 Antibody [NB100-53784] - (1ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FBXL3 Antibody Summary

• Description: Novus Biologicals Goat FBXL3 Antibody (NB100-53784) is a polyclonal antibody validated for use in WB and ELISA. Anti-FBXL3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence KRGGRDSDRNSSEE-C corresponding to N-Terminus according to NP_036290.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FBXL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50-55 kDa band observed in human adipose lysates (calculated MW of 48.7 kDa band according to NP_036290.1).
• Control:

  Human Adipose Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXL3 Antibody

• FBL3AF-box and leucine-rich repeat protein 3AF-box protein Fbl3a
• FBL3F-box/LRR-repeat protein 3
• F-box and leucine-rich repeat protein 3
• F-box/LRR-repeat protein 3A
• FBXL3A

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXL3 Antibody (NB100-53784)(2)

Publications using NB100-53784

• Korge S, Grudziecki A, Kramer A. Highly Efficient Genome Editing via CRISPR/Cas9 to Create Clock Gene Knockout Cells. J. Biol. Rhythms. 2015-08-04 [PMID: 26243628] (WB, Human)
  
  Details:
  FBXL3 antibody was used for WB analysis of total cell lysate (80 ug per lane) from either wild-type or FBXL3 RNAi-downregulated U2-OS cells. The antibody was able to recognize ectopically overexpressed hFBXL3 also.
• Albig AR, Neil JR, Schiemann WP. Fibulins 3 and 5 antagonize tumor angiogenesis in vivo. Cancer Res 2006-03-01 [PMID: 16510581]

Bioinformatics

• Gene Symbol: FBXL3
• Entrez:
  • 26224(Human)
• Uniprot:
  • NP_036290.1(Human)