FAM165B Overexpression Lysate (Native)

Images

 
Western Blot: FAM165B Overexpression Lysate (Adult Normal) [NBL1-10493] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FAM165B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FAM165B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223252 and based on accession number NM_058182. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens family with sequence similarity 165, member B (FAM165B), mRNA.
Gene
SMIM11

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
6.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FAM165B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FAM165B Overexpression Lysate (Native)

  • C21orf51
  • chromosome 21 open reading frame 51
  • family with sequence similarity 165, member B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-86818
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90676
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00054097-M07
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, IP
NBP1-86817
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88936
Species: Hu
Applications: IHC, IHC-P
NBP1-88279
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88706
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88280
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81767
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-14420
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-98259
Species: Hu
Applications: WB
NBP1-89876
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-85912
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBL1-10493
Species: Hu
Applications: WB

Publications for FAM165B Lysate (NBL1-10493) (0)

There are no publications for FAM165B Lysate (NBL1-10493).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FAM165B Lysate (NBL1-10493) (0)

There are no reviews for FAM165B Lysate (NBL1-10493). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FAM165B Lysate (NBL1-10493) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FAM165B Products

FAM165B NBL1-10493

Bioinformatics Tool for FAM165B Lysate (NBL1-10493)

Discover related pathways, diseases and genes to FAM165B Lysate (NBL1-10493). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on FAM165B

There are no specific blogs for FAM165B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SON Antibody
NBP1-88706
FAM3B Antibody (1E7)
H00054097-M07
GCFC1 Antibody
NBP1-86818

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FAM165B Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SMIM11