Exostosin 1 Overexpression Lysate

Images

 
Western Blot: Exostosin 1 Overexpression Lysate (Adult Normal) [NBL1-10391] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Exostosin 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Exostosin 1 Overexpression Lysate Summary

Description
Transient overexpression lysate of exostoses (multiple) 1 (EXT1) The lysate was created in HEK293T cells, using Plasmid ID RC200644 and based on accession number NM_000127. The protein contains a C-MYC/DDK Tag.
Gene
EXT1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
86.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Exostosin 1 Overexpression Lysate

  • EC 2.4.1.224
  • EC 2.4.1.225
  • exostoses (multiple) 1
  • Exostosin 1
  • exostosin-1
  • EXT
  • EXT1
  • Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
  • Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan4-alpha-N-acetylglucosaminyltransferase
  • Langer-Giedion syndrome chromosome region
  • LGCR
  • LGS
  • Multiple exostoses protein 1
  • N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase
  • Putative tumor suppressor protein EXT1
  • TRPS2
  • TTV

Background

This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Exostosin 1 Lysate (NBL1-10391) (0)

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-10391

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Bioinformatics

Gene Symbol EXT1