Novus Biologicals products are now on bio-techne.com

Exostosin 1 Overexpression Lysate

Images

 
Western Blot: Exostosin 1 Overexpression Lysate (Adult Normal) [NBL1-10391] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Exostosin 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Exostosin 1 Overexpression Lysate Summary

Description

Exostosin 1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200644

Accession#: NM_000127

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
EXT1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
86.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Exostosin 1 Overexpression Lysate

  • EC 2.4.1.224
  • EC 2.4.1.225
  • exostoses (multiple) 1
  • Exostosin 1
  • exostosin-1
  • EXT
  • EXT1
  • Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
  • Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan4-alpha-N-acetylglucosaminyltransferase
  • Langer-Giedion syndrome chromosome region
  • LGCR
  • LGS
  • Multiple exostoses protein 1
  • N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase
  • Putative tumor suppressor protein EXT1
  • TRPS2
  • TTV

Background

This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-58297
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-23603
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
AF2635
Species: Hu
Applications: WB
AF1705
Species: Mu
Applications: IHC, WB
NBP1-31225
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF6585
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
NBP2-16394
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB6734
Species: Hu
Applications: IHC
AF2364
Species: Hu
Applications: IHC, WB
233-FB
Species: Hu
Applications: BA
AF4838
Species: Hu
Applications: Simple Western, WB
H00003077-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
AF1329
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
H00002523-P01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB4214
Species: Mu
Applications: IP, WB

Publications for Exostosin 1 Lysate (NBL1-10391) (0)

There are no publications for Exostosin 1 Lysate (NBL1-10391).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Exostosin 1 Lysate (NBL1-10391) (0)

There are no reviews for Exostosin 1 Lysate (NBL1-10391). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Exostosin 1 Lysate (NBL1-10391) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Exostosin 1 Products

Research Areas for Exostosin 1 Lysate (NBL1-10391)

Find related products by research area.

Blogs on Exostosin 1

There are no specific blogs for Exostosin 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Exostosin 1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol EXT1