EVA1/MPZL2 Overexpression Lysate (Native)

Images

 
Western Blot: MPZL2 Overexpression Lysate (Adult Normal) [NBL1-13215] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MPZL2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EVA1/MPZL2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC223753 and based on accession number NM_144765. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens myelin protein zero-like 2 (MPZL2), transcript variant 2, mRNA.
Gene
MPZL2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EVA1/MPZL2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EVA1/MPZL2 Overexpression Lysate (Native)

  • EVA1
  • EVAEpithelial V-like antigen 1EVA1myelin protein zero-like protein 2
  • MPZL2
  • myelin protein zero-like 2

Background

Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. This gene is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1 and is the shorter transcript.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MPZL2
Entrez