Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free

Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Eukaryotic translation initiation factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and imm

Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Eukaryotic translation initiation factor 1.

Immunohistochemistry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Staining of paraffin-embedded Human lymph node tissue using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.

Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Analysis of Jurkat cells, using anti-Eukaryotic translation initiation factor 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Staining of HeLa cells using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.

Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Analysis of Hela cells, using anti-Eukaryotic translation initiation factor 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

CyTOF-ready: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Eukaryotic translation initiation factor 1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free (NBP2-00702) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human EIF1 (NP_005792) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 12.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free

• A121Protein translation factor SUI1 homolog
• eIF1
• EIF-1
• EIF1A
• eukaryotic translation initiation factor 1
• ISO1
• Sui1iso1
• SUI1sui1iso1

Background

EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF1