ERK3/MAPK6 Antibody (4C11)

Western Blot: ERK3/MAPK6 Antibody (4C11) [H00005597-M02] - MAPK6 monoclonal antibody (M02), clone 4C11 Analysis of MAPK6 expression in Jurkat.

Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (4C11) [H00005597-M02] - Analysis of monoclonal antibody to MAPK6 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: ERK3/MAPK6 Antibody (4C11) [H00005597-M02] - MAPK6 monoclonal antibody (M02), clone 4C11. Analysis of MAPK6 expression in PC-12.

Western Blot: ERK3/MAPK6 Antibody (4C11) [H00005597-M02] - MAPK6 monoclonal antibody (M02), clone 4C11. Analysis of MAPK6 expression in Raw 264.7.

Sandwich ELISA Capture: ERK3/MAPK6 Antibody (4C11) [H00005597-M02] - Detection limit for recombinant GST tagged ERK3/MAPK6 is 0.3 ng/ml as a capture antibody.

Product Discontinued

ERK3/MAPK6 Antibody (4C11) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPK6 (AAH35492, 612 a.a. ~ 721 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
• Specificity: MAPK6 - mitogen-activated protein kinase 6 (4C11)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK6
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA Capture
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERK3/MAPK6 Antibody (4C11)

• DKFZp686F03189
• EC 2.7.11
• EC 2.7.11.24
• ERK3
• ERK-3
• ERK3p97-MAPK
• Extracellular signal-regulated kinase 3
• extracellular signal-regulated kinase, p97
• HsT17250
• MAP kinase 6
• MAP kinase isoform p97
• MAPK 6
• MAPK6
• mitogen-activated protein kinase 6
• p97MAPK
• PRKM6
• protein kinase, mitogen-activated 5
• protein kinase, mitogen-activated 6

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK6
• OMIM:
  • 602904(Human)