EphB2 Antibody (512013)

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Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse EphB2 Monoclonal Antibody (Catalog # MAB4672) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
512013
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

EphB2 Antibody (512013) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB2
Val27-Lys548
Accession # P54763
Specificity
Detects mouse EphB2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human EphB2 is observed. In Western blots, no cross-reactivity with recombinant human EphB2 is observed. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) EphA1, 2, 3, 4, 5, 6, 7, 8, rmEphB4, 6, recombinant human EphA10, recombinant rat (rr) EphA10, or rrEphB1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
EPHB2
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB4672 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB2 Antibody (512013)

  • CAPB
  • Cek5
  • Drt
  • DRTEphB2
  • EC 2.7.10
  • EC 2.7.10.1
  • EK5
  • elk-related tyrosine kinase
  • EPH receptor B2
  • eph tyrosine kinase 3
  • EphB2
  • EPH-like kinase 5
  • ephrin type-B receptor 2
  • EPHT3MGC87492
  • EPTH3
  • Erk
  • ERKHek5
  • Hek5
  • Nuk
  • PCBC
  • protein-tyrosine kinase HEK5
  • Qek2
  • Renal carcinoma antigen NY-REN-47
  • Sek3
  • Tyro5
  • Tyrosine-protein kinase receptor EPH-3
  • Tyrosine-protein kinase TYRO5

Background

EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB2 Antibody (MAB4672)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Blogs on EphB2.

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By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Bioinformatics

Gene Symbol EPHB2
Uniprot