ENPP-1 Antibody


Western Blot: ENPP-1 Antibody [NB600-816] - Western blot analysis of ENPP-1 in HepG2 cell lysate at 2.0 ug/ml.
Immunohistochemistry-Paraffin: ENPP-1 Antibody [NB600-816] - ENPP1 Antibody [NB600-816] - ENPP1 antibody was tested in human liver using DAB with hematoxylin counterstain.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
1.18 mg/ml

ENPP-1 Antibody Summary

A synthetic peptide made to a C-terminal portion of the human ENPP1 protein (between residues 900-925) [UniProt P22413]
Immunogen affinity purified
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  • Western Blot 1:1000
  • Immunohistochemistry-Paraffin 1:200
  • Peptide ELISA 1:2000
Application Notes
This ENPP1 antibody is useful for Peptide ELISA, Western Blot and Immunohistochemistry-paraffin embedded sections. In Western blot a band was observed at ~130-150kDa in Human Liver and HepG2 lysates that was blocked by preincubation with the immunizing peptide. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ENPP-1 Lysate (NBL1-10273)
HepG2 Lysate (NBP2-03956)
Control Peptide
ENPP-1 Peptide (NB600-816PEP)

Reactivity Notes

Human. Immunogen sequence has 85% homology to mouse and rat.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
1.18 mg/ml
Immunogen affinity purified

Alternate Names for ENPP-1 Antibody

  • ectonucleotide pyrophosphatase/phosphodiesterase 1
  • E-NPP 1
  • ENPP1
  • ENPP-1
  • Ly-41 Antigen
  • M6S1
  • M6S1NPP1
  • Membrane component chromosome 6 surface marker 1
  • membrane component, chromosome 6, surface marker 1
  • NPP1
  • NPPS
  • NPPSalkaline phosphodiesterase 1
  • PC1
  • PC-1
  • PCA1
  • PDNP1
  • PDNP1ectonucleotide pyrophosphatase/phosphodiesterase family member 1
  • Phosphodiesterase I/nucleotide pyrophosphatase 1
  • plasma-cell membrane glycoprotein 1
  • Plasma-cell membrane glycoprotein PC-1


ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase family member 1) is a type II transmembrane glycoprotein which is implicated in membrane surface ATP hydrolysis, regulation of pyrophosphate levels, bone mineralization as well as soft tissue calcification. ENPP1 negatively regulates bone mineralization by hydrolyzing extracellular nucleotide triphosphates (NTPs) to produce inorganic pyrophosphate (PPi, an inhibitor of mineralization), whereas tissue-nonspecific ALP positively regulates mineralization by hydrolyzing NTPs and PPi to produce extracellular concentrations of inorganic phosphate (Pi, a substrate for mineralization). ENPP1 is expressed on outer surfaces of mineralizing cells, such as osteoblasts and chondrocytes, and on the membranes of osteoblast- and chondrocyte-derived matrix vesicles. It also hydrolyzes other NTPs such as GTP, CTP, TTP, UTP etc. to their corresponding monophosphates with the release of PPi and diadenosine polyphosphates, and also 3',5'-cAMP to AMP, and may also regulate the availability of nucleotide sugars in ER/Golgi complex, as well as the regulation of purinergic signaling. ENPP1 binds directly to INSR for inhibiting insulin signaling and ENPP1's K173Q/ K121Q polymorphism is associated with insulin resistance, NIDDM, and obesity. Defective ENPP1 causes increased susceptibility for ossification of the posterior longitudinal ligament of spine (OPLL), arterial calcification of infancy, generalized, type 1 (GACI1), obesity/glucose intolerance/NIDDM, and rickets hypophosphatemic autosomal recessive type 2 (ARHR2).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-816AF405
Alexa Fluor 488 Labeled NB600-816AF488
Alexa Fluor 647 Labeled NB600-816AF647
Alexa Fluor 700 Labeled NB600-816AF700
Biotin Labeled NB600-816B
DyLight 405 Labeled NB600-816V
DyLight 488 Labeled NB600-816G
DyLight 550 Labeled NB600-816R
DyLight 650 Labeled NB600-816C
FITC Labeled NB600-816F
HRP Labeled NB600-816H

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Gene Symbol ENPP1